According to the published ORF2 genes sequence of PCV-2 in GenBank, one pair of PCR primers were designed, and the nuclear localization signal (NLS)-defected capsid protein gene(d Cap)of porcine circovirus type 2 (PCV-2)was expressed. Then the PCR product was cloned into pEGX-4T-1vector by Bam HI and Xho I digestion and a recombinant plasmid named pEGX-4T-1-ORF2 was obtained, which was induced by IPTG, The expressive product was purified by the GST-Protein Purification Kit. The purification effect and the specificity of the purified recombinant capsid proteins were detected respectively by SDS-PAGE and Western blot assay. The results demonstrated that ORF2 gene was successfully?cloned, which was 579 bp; the size of rCap protein was 45.3 ku, which was in line with the expected size; there is only one purpose band of 45.3 ku in size with above 90% purity. The results show that the recombinant Cap have been well purified and could react with the polyclonal antibody against PCV-2, and possess good antigenicity.