A strain of virus was isolated from suspected duck hepatitis virus (DHV) in duckling liver samples through chicken embryo allantoic sac inoculation. The sample was collected from ducklings diagnosed clinically dying from duck virus hepatitis on the duck farm in Binzhou county of Shandong province in China. Animal test indicated that the death rate was 80％. The virus strain was identified as DHV serotypeⅠand named as DHV BZ-Ⅰstrain by observation of clinical performance and the tests results of pathological examination, physical-chemical characteristics, neutralization , RT-PCR , and animal pathogenicity. This study could provide a useful data for effective prevention of DVH.