Abstract: Salmonella spp. is an important kind of food-borne pathogenic bacteria which can cause human and animal disease. Salmonella invA gene is known as a major virulence gene. According to published Salmonella invA gene sequence in GenBank, we designed one sets of specific loop-mediated isothermal amplification primers and developed a novel and highly specific loop-mediated isothermal amplification assay for the sensitive and rapid detection of Salmonella spp. The assay correctly identified Salmonella spp., but did not detect 7 non-Salmonella spp. strains. Sensitivity of the LAMP asssay for direct detection of Salmonella spp. in pure cultures was 1.04×102 cfu·mL-1. The results of LAMP detection of 100 eggs were consistent with the traditional method. The LAMP assay is a sensitive, rapid and simple tool for the detection of Salmonella spp. and will facilitate the surveillance for control of contamination of Salmonella spp. in egg.
