In this paper,we aimed to establish an isolation method and culture system for Macaca fascicularis ear fibroblast cells in vitro by means of tissue block culture.Primary culture of Macaca fascicularis ear fibroblast cells could be obtained through tissue block culture successfully.Using 0.25% trypsin+0.02% EDTA as digested medium and DMEM containing 10% FBS as culture medium for subculture,the cells were passaged to the 25th passage,and then were frozen in DMEM medium containing 10% FBS and 10% DMSO with th...