The uptake patterns of different peptides in the culture by rumen microorgaisms were explored.The results showed that the amount of peptides uptake was increased significantly (P<0.01 or P<0.05) with incubation time extending,but the rates of peptides uptake was decreased significantly (P<0.01).When peptides concentration was increased in the incubation,the amount of peptides uptake and rates of peptides uptake were increased significantly (P<0.01 or P<0.05),but the proportion of peptides degradation was decreased significantly (P<0.05).The amount,proportion and rate of rumen peptides uptake were higher than those of soybean protein peptide (P<0.05) and corn protein peptide (P<0.01).The results suggested that the rate of peptides uptake might be different when they were from different proteins resources.