By rinsing, airing, comminuting, soaking, squeezing and filtrating the leaves, stems, roots and seeds from insect resistant transgenic cotton, the steeps were prepared.Experiments for controlling 3~(rd) instar larvae of Clostera anastomosis were conducted.The results showed that the steeps from 100 g fresh leaves dregs with 60 mL water and 30 g dry leaves powder with 60 mL water had good results on Clostera anastomosis control with mortalities of 93.3 % and 100%, respectively, in 2 hours. The mortalities of Clostera anastomosis were 76 % and 88%, respectively, in 5 days by the field willow treated with the steeps from 1000 g dry leaves powder with 5000 mL water and 1000g seed powder with 5000 mL water, respectively.