Coenzyme Q 10 and Cytochrome C are biochemical medicine of biooxidase.The artical has developed a method of extracting and purification of coenzyme Q 10 and cytochrome C from pig heart.Pig heart is ronghly treated in secondary acid liquid and 225 mg cytochrome C (purity 79%) from 1 kg pig heart is obtained through adsorption,eluting,salting out,precipitating,vacuum drying.75 mg coenzyme (Q 10 concentration 95 3%) is extracted from 1 kg pig heart residue through saponification,chromatography and crytallization.