CaCl2 was a better dissolvent than LiBr to dissolve silk fibroin.The silk fi-hroin membrane was successively made by silk fibroin solution casting in PVC plate at roomtemporeture under controlled huinidity after dialysised by distilled wate. Insolubiiizated bymethanol or glutaraldehyde the silk fibroin membrane thus made provtJd to be fq good carrierfor immobilized enzvmes.