The distinct changes of esterase isozyme and peroxidase isozyme in numbers,kinds and activities were observed after N+ ion with 30 kev energy implanting into branch-bud of Populus deltoides and embry of Cinkgo biloba. The results demonstrated that the isozyme activities became weaker with the doses increased.The physiological variations were inheritable through vegetative procesesses. Besides,N+ ion implantation caused significant decrease of field sprouting percentage,and the semilethal doses effects showed a non-index change.