Calf diarrhoea, a common disease in the north of Huanghe River in both Anhui and Henan provinces since 1981, has been etiologically unknown. The present study demonstrates it to be a rotaviral diseazse, with a detection rate of 48.33% by TEM. Of 105 excrement samples, 34 wese detected as positive by RIHA with detection rate at 37.1%. Typical rotavirus nucleic acid electrophorsis atlas were observed among 23 of 48 excrement samples by PAGE The coincidencerate of the 2 methods was 91.7%. Rotavirus antibodies were found ni 182 of 210 female cattle serum Samples by RIHI. Four neonatal calves which were nioculated with cattle rotavirus adapted in cell through mouth niduced clinical symptoms from 41.5 hours after nioculation. The cure rates were 95.27%, and 91.89% respectively, by experimental catttle gamma globulin and cattle serum interferon treatments.